Mazoyères-Chambertin is an Appellation d'origine contrôlée and Grand Cru vineyard for red wine in the Côte de Nuits subregion of Burgundy, with Pinot noir as the main grape variety. Mazoyères-Chambertin is located within the commune of Gevrey-Chambertin. It is situated below the Route des Grands Crus. It borders on Latricières-Chambertin and the Gevrey-Chambertin Premier Cru aux Combottes in the west, Charmes-Chambertin in the north, Gevrey-Chambertin village level vineyards in the east, and Morey-Saint-Denis Premier Cru vineyards in the south. The AOC was created in 1937.
A peculiarity of Mazoyères-Chambertin is that the appellations Charmes-Chambertin (of the neighbouring Grand Cru vineyard) may be used interchangeably with that of Mazoyères-Chambertin. As Charmes-Chambertin is the more popular to use, relatively little wine is bottled as Mazoyères-Chambertin, which is only around 10 per cent of the production.
Another grand cru vineyard in Gevrey Chambertin with a similar name, but no other connection is Mazis-Chambertin which is also known as Mazy-Chambertin.